Dissertação/Tese DIVERSIDADE MICROBIANA EM SUBSTRATOS DESCARTADOS DURANTE AS FASES DO PROCESSO DE PRODUÇÃO DE MUDAS CLONAIS DE EUCALIPTO - PPGCF

A produção de muda é uma etapa primordial e decisiva para a implantação de uma floresta. No entanto, devido a diversos fatores de ordem técnica, as perdas no setor de produção são bastante relevantes. Dentre estes fatores está o descarte do substrato, que

The production of changes is a crucial and decisive step for the implementation of a forest. However, due to several technical factors, losses in the production sector are quite relevant. Among these factors is the disposal of substrate, which when done wrongly favors the proliferation of phytopathogenic microorganisms in nursery. This can trigger significant losses and still become a environmental liabilities. Rarely the substrate is reused in commercial nurseries, since it is assumed that the physical, chemical and biological characteristics were lost during the production of seedlings. However, there are few studies on microorganisms on substrates. The importance of this study is based on the scarcity of research on this issue that affects the production of seedlings, as well as seek sustainable alternatives that assumes the use of this raw material demand. The application of molecular techniques have been employed to detect and identify microorganisms in various environments. In this context, the objective of this study was to detect microbiological diversity in substrate samples dropped by PCR-RFLP technique. Ten samples were collected of substrates in different nurseries in the State of Minas Gerais in different stages of production and State of modern farms. Total genomic DNA was extracted and amplified by the PCR technique with specific oligonucleotides to fungi, bacteria and archaea. The amplified products were submitted to cleavage with HaeIII restriction enzymes, BamHI, HindIII, and TaqI for detection of possible polymorphisms between the samples by PCR-RFLP technique. It was possible to check differences between samples of substrates, both in relation to the size of the amplified DNA region as well as in relation to the presence of restriction sites. Based on the index of similarity, if a greater variation of diversity within the sample at different stages than in samples between different nurseries. Therefore, these results may be relevant to the knowledge of microbiological diversity in substrate. However more studies are needed to better understanding of these microorganisms and their role in the substrate.